Composite

Part:BBa_J56017:Design

Designed by: Matt Eames   Group: iGEM06_UCSF   (2006-10-31)


sequence containing necessary DNA for controlled chemotaxis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 7395
    Illegal SpeI site found at 2824
    Illegal PstI site found at 3761
    Illegal PstI site found at 4213
    Illegal PstI site found at 5336
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1300
    Illegal NheI site found at 5488
    Illegal SpeI site found at 2824
    Illegal PstI site found at 3761
    Illegal PstI site found at 4213
    Illegal PstI site found at 5336
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1239
    Illegal XhoI site found at 5695
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 7395
    Illegal SpeI site found at 2824
    Illegal PstI site found at 3761
    Illegal PstI site found at 4213
    Illegal PstI site found at 5336
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 7395
    Illegal SpeI site found at 2824
    Illegal PstI site found at 3761
    Illegal PstI site found at 4213
    Illegal PstI site found at 5336
    Illegal NgoMIV site found at 5637
    Illegal NgoMIV site found at 6564
    Illegal AgeI site found at 1074
    Illegal AgeI site found at 1340
    Illegal AgeI site found at 3283
    Illegal AgeI site found at 5314
    Illegal AgeI site found at 7017
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1056
    Illegal SapI.rc site found at 5846


Design Notes

CheW* (VM108), Tar receptor codon-optimized to avoid recombination.


Source

Synthesized by DNA 2.0

References